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Creators/Authors contains: "Kanygin, Andrey"

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  1. ; ; ; ; ; ; ; (, Energy & Environmental Science)
    Harnessing the power of photosynthesis to catalyze novel light-driven redox chemistry requires a way to intercept electron flow directly from the photosynthetic electron transport chain (PETC). As a proof of concept, an in vivo fusion of photosystem I (PSI) and algal hydrogenase was created by insertion of the HydA sequence into the PsaC subunit. The PSI and hydrogenase portions are co-assembled and active in vivo , effectively creating a new photosystem. Cells expressing only the PSI-hydrogenase chimera make hydrogen at high rates in a light-dependent fashion for several days. In these engineered cells, photosynthetic electron flow is directed away from CO 2 fixation and towards proton reduction, demonstrating the possibility of driving novel redox chemistries using electrons from water splitting and the photosynthetic electron transport chain. 
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  2. ; ; ; ; ; ; ; ; (, Biochimica et Biophysica Acta (BBA) - Bioenergetics)
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